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Companion In Vivo Imaging Diagnostics

When substituting HRPs or drug molecules with radiolabels such as Tc–99m, In–111, Gallium–68 or Gadolinium, the Z-TECT™ Carrier Unit becomes an imaging unit for in vivo applications such as Gamma imaging (Figures 1 and 2), Single Photon Emission Computed Tomography (SPECT), Positron Emission Tomography (PET) and Magnetic Resonance Imaging (MRI).

Figure 1 shows the efficiency of the Z-TECT™ Targeting Unit by comparing images with and without pre-targeting.

Figure 2 shows the efficiency and ultrasensitivity of the Z–TECT™ Carrier Unit by clearly identifying a “hot” region pointed by the white arrow which corresponds to about 4 μg of surrogate antigen-coated microbeads, even with limited camera resolution.

Figure 1. Gamma anteroposterior images (99m–Tc) of mice with MCF-7 fibrosarcoma pre-targeted with Z-TECT™ Targeting Unit (left) or not pre-targeted (right).

The next day, the Z-TECT™ Carrier Unit was injected i.v. and images were obtained at 6 hrs.  The circle (top) is 1/20th of injected dose.

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Figure 1. Gamma anteroposterior images (99m–Tc) of mice with MCF-7 fibrosarcoma pre-targeted withZ–TECT™ Targeting Unit (left) or not pre–targeted (right). The next day, Z–TECT™Imaging Unit was injected i.v. and images were obtained at 6 hrs. The circle (top) is 1/20th of injected dose.

Figure 2. Gamma image (99m–Tc) of surrogate antigen–coated microbeads of 4 μg in size at 24 hrs (L, K and B indicate liver, kidneys and bladder).

For more information about companion in vivo imaging applications based on Akrivis' Z-TECT™ technology platform, please contact us at info@akrivis.com.